ANTIBACTERIAL ACTION OF SILVER NANOPARTICLES

Objective. To determine the minimum inhibitory concentration of Ag nanoparticles in relation to clinical pathogenic strains microorganisms. Methods. The nanoparticles, obtained by metal vapor synthesis was studied on six bacteria, including representatives gram-positive and gram-negative groups. microbiological analyzer Vitek 2 Compact used identify each strain antibiogram. study were synthesized method metalvapor synthesis. transmission electron microscopy(TEM) X-ray photoelectron spectroscopy (XPS) methods. Determination performed serial dilution using sterile 96-well plates with tests positive negative control. microorganisms controlled turbidity standard. Results. All bacteria characterized pronounced polyantibiotic resistance, percentage antibiotics against which resistant ranged from 12.5 93.3%. silver 7.81 31.25 μg/ml, depending type microorganism. Gram-positive microorganisms, contrast ones, lower values concentration. data showed that size is range 2-15 nm. Conclusion. Silver (2-15 nm size) have antimicrobial action clinically significant, polyantibiotic-resistant microorganism, varies μg/ml. an impact a greater extent inhibit growth versus gram-negative. materials based represent effective alternative currently antibacterial drugs. What this paper adds quantitative indices been firstly polyantibioticresistant resistance does not affect degree these has demonstrated considered be significant step development scientifically class substances surgery